Fig. 5

Simulation of 6 channels over a band of 43.5 kHz; 8.7 kHz separation between neighboring channels. The arrangement of six 20 μm diameter simulated beads in six different channels is shown in (a). A simulated SRS image of a bead with time constant of 26.5 μs and filter order 8 in channel 3 is shown in (b). In (c) the same channel is simulated with a time constant of 1.6 μs and filter order 8, showing crosstalk between channel 3 and the rest of the channels as a modulation that depends on their frequency difference. Channel 3 was also simulated with a time constant of 125 μs and filter order 1 (d), which results in the same NEPBW as the parameters used for b. Despite the same NEPBW, there is much more crosstalk between the different channels due to the filter shape. Scale bars: 20 μm